SUBCLONING OF THE STREPTOKINASE GENE FROM STREPTOCOCCUS PYOGENES INTO pCold I AND pCold TF PLASMIDS

Authors

  • Vokhidov Kh. 1 Автор
  • Kholikov A. 1 Автор
  • Alimova B.Kh. 1 Автор
  • Makhsumkhanov A.A. 1 Автор

Abstract

Currently, the demand for thrombolytic drugs is continuously increasing. Pharmaceutical preparations based on the recombinant form of streptokinase are distinguished by their cost-effectiveness compared to other thrombolytic agents (Diwan et al., 2021). The nucleotide sequence of the streptokinase (Ska) gene, isolated from the bacterium Streptococcus pyogenes, is registered in the international GenBank database under the accession number PX644861.1. Initially, this gene was cloned into the pET-28a(+) plasmid (Vokhidov et al., 2025), and its expression characteristics were studied in E. coli host cells. This study presents the results of subcloning the Ska gene into pCold TF and pCold I vectors to enhance the solubility and yield of the recombinant protein in subsequent expression stages (Bhatwa et al., 2021; Qing et al., 2004).. 

 

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Published

2026-06-24

Issue

Vol. 2 No. 6 (2026)

Section

Articles

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How to Cite

Vokhidov, K., Kholikov , A., Alimova , B., & Makhsumkhanov, A. (2026). SUBCLONING OF THE STREPTOKINASE GENE FROM STREPTOCOCCUS PYOGENES INTO pCold I AND pCold TF PLASMIDS . International Conference on Social Sciences & Humanities, 2(6), 160-162. https://uniconflix.com/index.php/ICSH/article/view/5347