PCR, ANAEROBIC BACTERIA, MAXILLOFACIAL INFLAMMATION, MICROBIOCENOSIS, REAL-TIME PCR, MOLECULAR DIAGNOSTICS
DOI:
https://doi.org/10.5281/zenodo.18832435Abstract
In recent years, traditional microbiological diagnostic methods for inflammatory diseases have been supplemented by molecular genetic technologies. Polymerase chain reaction (PCR), developed in the mid-1980s, enables rapid DNA amplification and allows identification of microorganisms without isolation of pure cultures. This study aimed to improve the etiological diagnosis of inflammatory diseases of the maxillofacial region using real-time PCR. The results demonstrated frequent detection of anaerobic pathogens and significant disruption of the natural microbiocenosis with the predominance of opportunistic flora. Real-time PCR proved to be a highly informative and specific method for detecting difficult-to-cultivate anaerobic bacteria.
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